2161 - 生物化學實驗

Biochemistry Lab.

教育目標 Course Target

使學生藉由實驗操作更加了解蛋白質,醣類,脂質及DNA之基本特色透過實驗的操作,使學生的生化知識理論能更加清晰,課程內容設計主要有胺基酸的定性、蛋白質定量、蛋白質電泳、酵素水解、質體DNA萃取、PCR反應及洋菜膠電泳,一系列的課程使學生能藉由動手做,更能了解。

Through experiment operations, students can better understand the basic characteristics of protein, sugar, lipid and DNA. Through experiment operations, students' biochemical knowledge theory can be clearer. The course content design mainly includes qualitative amino acids, protein quantification, protein electrophoresis, enzyme hydrolysis, quality DNA extraction, PCR reaction and watercress electrophoresis. A series of courses enable students to understand better through manual work.

課程概述 Course Description

透過實驗的操作,使學生的生化知識理論能更加清晰,課程內容設計主要有胺基酸的定性、蛋白質定量、螢光蛋白純化、蛋白質電泳、酵素水解、基因轉殖、質體DNA萃取、限制酵素剪切、PCR反應及洋菜膠電泳,一系列的課程使學生能藉由動手做,更能了解。

Through the experiment, students' biochemical knowledge theory can be clearer. The course content design mainly includes qualitative amino acids, protein quantification, photoprotein purification, protein electrophoresis, enzyme hydrolysis, gene regeneration, quality DNA extraction, restricted enzyme shear, PCR reaction and seaweed gel electrophoresis. A series of courses enable students to do it manually and understand better.

參考書目 Reference Books

生物化學實驗原理和方法 李建武等合著 藝軒出版社
生物化學實驗書 柯文慶 賴滋漢等合著 富林出版社
其他見數位平台

Principles and methods of biochemistry experiments co-authored by Li Jianwu and others Art Publishing House
Biochemistry experiment book Co-authored by Ke Wenqing, Feng Zihan and others Fulin Publishing House
Other digital platforms

評分方式 Grading

評分項目
Grading Method
配分比例
Percentage
說明
Description
報告作業
Reporting
30 含實驗結果25%、影片報告作業5%
出席
Attend
15 穿著實驗衣、報告按時繳交、實驗時用心與否
期末考
Final exam
25 筆試
口頭報告
Oral Report
15 分組口頭報告
跑檯
Run
15

授課大綱 Course Plan

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課程資訊 Course Information

基本資料 Basic Information

  • 課程代碼 Course Code: 2161
  • 學分 Credit: 1-0
  • 上課時間 Course Time:
    Thursday/5[AG122] Thursday/6,7[AG216]
  • 授課教師 Teacher:
    盧錫祺
  • 修課班級 Class:
    食科系3A
  • 選課備註 Memo:
    需同時修習或修過正課 第一堂課務必出席分組 未到者不受理加選。上課地點:AG216
選課狀態 Enrollment Status

目前選課人數 Current Enrollment: 40 人

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