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2185 - 微生物學實驗(一) Microbiology Lab (I)


教育目標 Course Target

初學者藉由顯微鏡(Microscope)的使用,以及細菌的簡單染色(simple staining),革蘭式染色(Gram staining)...等染色技術搭配顯微鏡觀察,幫助學生對微生物的型態,大小及結構的了解. 由培養基(medium)的製備及培養微生物的轉接技術(culture transfer),使學生學習如何培養細菌. 由分離技術(Isolation)及序列技術(Serial Dilution)了解檢驗微生物的方法. 由微生物的營養需求條件(pH, temp,培養基成分及氧氣)觀察微生物的生長情形. 由長時間的培養細菌以分光光度技去測量細菌生長情形而完成細菌生長曲線,以了解細菌複製世代所需的時間 Beginners use microscopes, simple staining, Gram staining, etc. to help students with microscope observations to help students in the shape, size and structure of microorganisms. Understanding. The preparation of medium and the culture transfer of culture to cultivate microorganisms enable students to learn how to cultivate bacteria. Learn how to test microorganisms through separation technology (Isolation) and Serial Dilution. The growth of microorganisms is observed by the nutrient requirements conditions (pH, temp, cultivation base components and oxygen). The bacterial growth curve is completed by measuring bacterial growth over a long period of time by spectrophotometry to understand the time required for bacterial reproduction generation.


課程概述 Course Description

Part1,on microscopy,introduces the use and care of the microscope for the study of microorganisms. Part2, on basis laboratory techniques for isolation, cultivation,and cultural characterization of microorganisms introduces basic procedures used for isolation and cultivation of micoorganisms. Part3,on bacterial staining, focuses on procedures for bacterial smear preparation, visualization, and differentiation of microorganisms and cell structures Part4, focuses on cultivation of microorganisms, nutritional and physical requirement, and enumeration of microbial populations.
Part1,on microscopy,introduces the use and care of the microscope for the study of microorganisms. Part2, on basis laboratories techniques for isolation, culture, and cultural characteristics of microorganisms introductions basic procedures used for isolation and culture of microorganisms. Part3,on bacterial staining, focuss on procedures for bacterial smear preparation, visualization, and differentiation of microorganisms and cell structures Part4, focuss on culture of microorganisms, nutritional and physical requirement, and enumeration of microbial populations.


參考書目 Reference Books

Textbook: Microbiology-A Laboratory Manual, 2015, 12th ed., J.G. Cappuccino, N. Sherman.
Microbiology-An Introduction, 2015, 11th ed. Tortora, G. T., Funke, B. R., and Case, C.L., Benjamin Cummings, 偉明圖書公司代理

Textbook: Microbiology-A Laboratory Manual, 2015, 12th ed., J.G. Cappuccino, N. Sherman.
Microbiology-An Introduction, 2015, 11th ed. Tortora, G. T., Funke, B. R., and Case, C.L., Benjamin Cummings, Agent of Via Ming Books


評分方式 Grading

評分項目 Grading Method 配分比例 Grading percentage 說明 Description
出席率(presence) 出席率(presence)
Attendance
15 每堂課開始都會點名
實驗報告(report)實驗報告(report)
Experimental Report (report)
30 實驗前撰寫預報 學習把實驗完成寫下實驗原理目的步驟及實驗結果
期末試驗(final test)期末試驗(final test)
Final test
25 測驗學生學習成效
實作評量(performance assessment)實作評量(performance assessment)
Performance assessment
20 測驗學生操作成效
實驗態度(experimental attitude) 實驗態度(experimental attitude)
Experimental attitude
10 鼓勵學生積極學習

授課大綱 Course Plan

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相似課程 Related Course

必修-2182 Microbiology Lab (I) / 微生物學實驗(一) (食科系1A,授課教師:陳永如,二/2,3,4[AG115])

Course Information

Description

學分 Credit:0-1
上課時間 Course Time:Wednesday/2,3,4[AG115]
授課教師 Teacher:謝陸盛
修課班級 Class:食科系1B
選課備註 Memo:需同時修習或修過正課 第一堂課務必出席分組 未到者不受理加選。上課地點:AG115
授課大綱 Course Plan: Open

選課狀態 Attendance

There're now 56 person in the class.
目前選課人數為 56 人。

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