初學者藉由顯微鏡(Microscope)的使用,以及細菌的簡單染色(simple staining),革蘭式染色(Gram staining)...等染色技術搭配顯微鏡觀察,幫助學生對微生物的型態,大小及結構的了解.
由培養基(medium)的製備及培養微生物的轉接技術(culture transfer),使學生學習如何培養細菌.
由分離技術(Isolation)及序列技術(Serial Dilution)了解檢驗微生物的方法.
由微生物的營養需求條件(pH, temp,培養基成分及氧氣)觀察微生物的生長情形.
由長時間的培養細菌以分光光度技去測量細菌生長情形而完成細菌生長曲線,以了解細菌複製世代所需的時間
Beginners can help students understand the shape, size and structure of microorganisms through the use of a microscope, as well as simple staining, Gram staining... and other staining techniques combined with microscope observation. understanding.
Students learn how to cultivate bacteria through the preparation of medium and the culture transfer technology for cultivating microorganisms.
Understand the methods of testing microorganisms through isolation technology (Isolation) and sequence technology (Serial Dilution).
Observe the growth of microorganisms based on their nutritional requirements (pH, temp, medium components and oxygen).
By culturing bacteria for a long time and measuring bacterial growth using spectrophotometric techniques, a bacterial growth curve is completed to understand the time required for bacterial replication generations.
Part1,on microscopy,introduces the use and care of the microscope for the study of microorganisms. Part2, on basis laboratory techniques for isolation, cultivation,and cultural characterization of microorganisms introduces basic procedures used for isolation and cultivation of micoorganisms. Part3,on bacterial staining, focuses on procedures for bacterial smear preparation, visualization, and differentiation of microorganisms and cell structures
Part4, focuses on cultivation of microorganisms, nutritional and physical requirement, and enumeration of microbial populations.
Part1, on microscopy, introduces the use and care of the microscope for the study of microorganisms. Part2, on basis laboratory techniques for isolation, cultivation, and cultural characterization of microorganisms introduces basic procedures used for isolation and cultivation of micoorganisms. Part3, on bacterial staining, focuses on procedures for bacterial smear preparation, visualization, and differentiation of microorganisms and cell structures
Part4, focuses on cultivation of microorganisms, nutritional and physical requirements, and enumeration of microbial populations.
Textbook: Microbiology-A Laboratory Manual, 2015, 12th ed., J.G. Cappuccino, N. Sherman.
Microbiology-An Introduction, 2015, 11th ed. Tortora, G. T., Funke, B. R., and Case, C.L., Benjamin Cummings, 偉明圖書公司代理
Textbook: Microbiology-A Laboratory Manual, 2015, 12th ed., J.G. Cappuccino, N. Sherman.
Microbiology-An Introduction, 2015, 11th ed. Tortora, G. T., Funke, B. R., and Case, C.L., Benjamin Cummings, Distributor of Weiming Books
| 評分項目 Grading Method | 配分比例 Grading percentage | 說明 Description |
|---|---|---|
| 出席率(presence) 出席率(presence) attendance(presence) |
15 | 每堂課開始都會點名 |
| 實驗報告(report)實驗報告(report) Experiment report(report) |
30 | 實驗前撰寫預報 學習把實驗完成寫下實驗原理目的步驟及實驗結果 |
| 期末試驗(final test)期末試驗(final test) final test |
25 | 測驗學生學習成效 |
| 實作評量(performance assessment)實作評量(performance assessment) performance assessment |
20 | 測驗學生操作成效 |
