使學生藉由本課程學習不同生物化學實驗操作。藉由生物化學實驗操作,瞭解生物化學專業知識之原理及應用,充實研究實力.This course enables students to learn different biochemical experimental operations. Through biochemical experimental operations, you can understand the principles and applications of biochemical professional knowledge and enrich your research capabilities.
透過實驗的操作，使學生的生化知識理論能更加清晰，課程內容設計主要有胺基酸的定性、蛋白質定量、螢光蛋白純化、蛋白質電泳、酵素水解、基因轉殖、質體DNA萃取、限制酵素剪切、PCR反應及洋菜膠電泳，一系列的課程使學生能藉由動手做，更能了解。
Through the operation of experiments, students' biochemical knowledge and theory can be made clearer. The course content is mainly designed to include the characterization of amino acids, protein quantification, fluorescent protein purification, protein electrophoresis, enzyme hydrolysis, gene transfer, plastid DNA extraction, and restriction. Enzyme shearing, PCR reaction and gel electrophoresis, a series of courses enable students to understand better through hands-on practice.
1. 生物化學實驗講義
2. Daniel,L.J.&A.L.Neal, “ Laboratory Experiments in Biochemistry ” , 1967
3. Hawk,P.B., “ Practical Physiogical Chemistry “ , 13th ed. , 1954 ,
The Blaskiston Company , Inc., New York
4. 生物化學實驗 , 第三版 , 環球書社 ( 丁一倪 , 何芳陔 , 張為憲 )
5. 生物化學實驗原理和方法 , 藝軒出版社 ( 李建武 , 余瑞元 等 )
1. Biochemistry Experiment Handouts
2. Daniel, L.J.&A.L.Neal, “ Laboratory Experiments in Biochemistry ”, 1967
3. Hawk,P.B., "Practical Physiogical Chemistry", 13th ed., 1954,
The Blaskiston Company, Inc., New York
4. Biochemistry Experiments, Third Edition, Global Books (Ding Yini, He Fangyi, Zhang Weixian)
5. Principles and methods of biochemical experiments, Yixuan Publishing House (Li Jianwu, Yu Ruiyuan, etc.)
| 評分項目 Grading Method | 配分比例 Grading percentage | 說明 Description |
|---|---|---|
| 實驗報告實驗報告 Experiment report |
50 | 實驗前預報、實驗後結報 |
| 實驗態度及實驗操作實驗態度及實驗操作 Experimental attitudes and experimental operations |
30 | 實驗操作的技巧與態度 |
| 學期考試學期考試 Semester exam |
20 | 實驗考試成績 |
