使學生藉由本課程學習不同生物化學實驗操作。藉由生物化學實驗操作,瞭解生物化學專業知識之原理及應用,充實研究實力.Let students learn different biochemistry experiments through this course. Through biochemistry experiments, we can understand the principles and applications of professional biochemistry knowledge and enrich our research capabilities.
透過實驗的操作，使學生的生化知識理論能更加清晰，課程內容設計主要有胺基酸的定性、蛋白質定量、螢光蛋白純化、蛋白質電泳、酵素水解、基因轉殖、質體DNA萃取、限制酵素剪切、PCR反應及洋菜膠電泳，一系列的課程使學生能藉由動手做，更能了解。
Through experiments, students' biochemical knowledge theory can be clearer. The course content design mainly includes qualitative amino acids, protein quantification, photophoretic protein purification, protein electrophoresis, enzyme hydrolysis, gene regeneration, quality DNA extraction, and restriction. Enzyme shear, PCR reaction and watercress electrophoresis, a series of courses allow students to do it manually and understand better.
1. 生物化學實驗講義
2. Daniel,L.J.&A.L.Neal, “ Laboratory Experiments in Biochemistry ” , 1967
3. Hawk,P.B., “ Practical Physiogical Chemistry “ , 13th ed. , 1954 ,
The Blaskiston Company , Inc., New York
4. 生物化學實驗 , 第三版 , 環球書社 ( 丁一倪 , 何芳陔 , 張為憲 )
5. 生物化學實驗原理和方法 , 藝軒出版社 ( 李建武 , 余瑞元 等 )
1. Biochemistry experiment explanation
2. Daniel, L.J.&A.L.Neal, “ Laboratory Experiments in Biochemistry ” , 1967
3. Hawk,P.B., “ Practical Physiogical Chemistry “ , 13th ed. , 1954 ,
The Blaskiston Company , Inc., New York
4. Biochemistry experiment, third edition, Global Book Club (Ding Yini, He Fangyan, Zhang Huanying)
5. Principles and methods of biochemistry experiments, Art Publishing House (Li Jianwu, Yu Ruiyuan, etc.)
| 評分項目 Grading Method | 配分比例 Grading percentage | 說明 Description |
|---|---|---|
| 實驗報告實驗報告 Experimental Report |
50 | 實驗前預報、實驗後結報 |
| 實驗態度及實驗操作實驗態度及實驗操作 Experience attitude and experience operation |
30 | 實驗操作的技巧與態度 |
| 學期考試學期考試 Period exam |
20 | 實驗考試成績 |
