本課程將以小腦脊髓運動失調症的三型疾病基因(ataxin-3)作為貫穿課程操作原理及實驗的模板，配合以實驗及實作練習，將課程的三大主題設計成實作單元，實作的結果分析將與過去的研究經驗實例比較。引導修課學生在基因操作的基本觀念建立後，以團體導向學習及討論方式，並加入動手練習，並配合過去的研究經驗實例演示，期使大學部學生了解如何以基因操作技術學來探討科學實務問題的本質。內容包含基因選殖基本方法，各類選殖載體，膠體電泳，DNA/RNA分析方法，聚合酶連鎖反應，定序與次世代定序等。This course will use the type 3 disease gene (ataxin-3) of cerebellospinal dyskinesia as a template for the operating principles and experiments throughout the course. With experiments and practical exercises, the three major themes of the course will be designed into practical units. The analysis of the results will be compared with examples of past research experience. After the basic concepts of gene manipulation are established, students will be guided to use group-oriented learning and discussion, and hands-on exercises will be added, as well as past research experience examples to demonstrate, with the aim of enabling undergraduate students to understand how to use gene manipulation technology to explore science. the nature of practical problems. The content includes basic methods of gene selection, various types of selection vectors, colloidal electrophoresis, DNA/RNA analysis methods, polymerase chain reaction, sequencing and next-generation sequencing, etc.
本課程將以基因操作實驗原理，配合以實驗方法與結果分析，來表示基因操作的基本觀念，以分組學習及討論方式，並期使大學部學生了解如何以基因工程技術學來探討科學的本質。內容包含基因選殖基本方法，各類載體選，膠體電泳，南方及北方點墨法，基因定序等。
This course will use the experimental principles of genetic manipulation, combined with experimental methods and result analysis, to present the basic concepts of genetic manipulation, through group learning and discussion, and aims to enable undergraduate students to understand how to use genetic engineering technology to explore the nature of science. . The content includes basic methods of gene selection and breeding, selection of various vectors, colloidal electrophoresis, southern and northern dot ink methods, gene sequencing, etc.
Gene Cloning and DNA analysis, T.A. Brown, 7th edition
Gene cloning and DNA analysis, T.A. Brown, 7TH edition
| 評分項目 Grading Method | 配分比例 Grading percentage | 說明 Description |
|---|---|---|
| Mid termMid term midterm |
35 | |
| 學習單作業學習單作業 worksheet homework |
25 | |
| 單元及期末報告單元及期末報告 Unit and period-end reports |
30 | |
| 出席與學習態度出席與學習態度 Attitude to Attendance and Learning |
10 |
