2116 - 生物化學實驗

Biochemistry Lab.

教育目標 Course Target

導引學生如何完成有機化學反應,終止反應操作,包括淬取及快速管柱層析,進而得到產物。使學生學習正確的實驗邏輯觀念,從失敗的實驗找出問題,進一步克服困難。再與課堂上教授的反應進一步與實驗結合,讓學生能學以應用。
1.Crystallization
2.Extraction(acid / base extraction)
3.Chromatography(Thin-Layer and Column Chromatography)
4.Conversion of Alcohols to Halides(Preparation of n-Butyl Bromide)
5.Williamson Ether Synthesis(Preparation of n-Butyl Methyl Ether)
6.Resolution of racemic phenylsuccinic acidusing(-)Proline as a resolving agent
7.Conversion of Alcohols to Alkenes(Preparation of Cyclohexene)
8.Nitration of Methyl Benzoate
9.Aldehydes and Ketones
10.Fries Rearrangement Accelerated by Microwave Radiation

Guide students to complete organic chemistry reactions, end reaction operations, including quenching and rapid column analysis, and then obtain products. Enable students to learn correct experimental logic concepts, find problems from failed experiments, and further overcome difficulties. It is further combined with the professor's response in class and experiments to allow students to learn and apply.
1.Crystallization
2.Extraction(acid/base extraction)
3.Chromatoography (Thin-Layer and Column Chromatoography)
4.Conversion of Alcohols to Halides(Preparation of n-Butyl Bromide)
5. Williamson Ether Synthesis(Preparation of n-Butyl Methyl Ether)
6.Resolution of racemic phenylsuccinic acidusing(-)Proline as a resolving agent
7.Conversion of Alcohols to Alkenes(Preparation of Cyclohexene)
8.Nitration of Methyl Benzoate
9.Aldehydes and Ketones
10.Fries Rearrangement Accelerated by Microwave Radiation

課程概述 Course Description

本課程內容有配製緩衝液、胺基酸之酸鹼滴定、半乳糖之米式常數、各種醣類定性、測定糞便中的脂肪、薄色層分析、蛋白質定量、蛋白質純化、親和層析分析及ELASA等實驗。

The course includes preparation of buffering, acid titration of amino acids, rice-forming normalization of galactose, qualitative qualitative properties of various types of sugars, detection of fat in stool, thin color analysis, protein quantification, protein purification, triglyceride analysis, and ELASA.

參考書目 Reference Books

1.Stenesh, J. 1984. Experimental Biochemistry. Allyn and Bacon. Boston.
2.Chaykin and Sterling. 1966. Biochemistry laboratory techniques. Wiley. New York.

1.Stenesh, J. 1984. Experimental Biochemistry. Allyn and Bacon. Boston.
2.Chaykin and Sterling. 1966. Biochemistry laboratories techniques. Wiley. New York.

評分方式 Grading

評分項目
Grading Method
配分比例
Percentage
說明
Description
實習報告
Actual Report
50
實習期末測驗
Actual final test
30
出席狀況、實驗態度
Attendance status, experience
20

授課大綱 Course Plan

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課程資訊 Course Information

基本資料 Basic Information

  • 課程代碼 Course Code: 2116
  • 學分 Credit: 0-1
  • 上課時間 Course Time:
    Tuesday/7,8,9[AG208]
  • 授課教師 Teacher:
    陳珠亮/羅能文
  • 修課班級 Class:
    畜產系2A
選課狀態 Enrollment Status

目前選課人數 Current Enrollment: 48 人

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