8305 - 植物組織培養學

Plant Tissue Culture

教育目標 Course Target

第1週 課程介紹
第2週 實驗室配備的介紹(生產計畫、培養之植物材料)
第3週 基本藥劑的準備(大量元素、微量元素、pH的調整、有機酸物之補充、糖)
第4週 無菌操作台之操作。(去汙染、表面消毒、減少表面汙染)
第5週 培養基的準備及使用(培養基、鹽類)
第6週 影響生長及形態發生的因素-組織依據的因素(培植體的年齡)
第7週 汙染及病害之控制(真菌及細菌病害,污染的類型)
第8週 無病毒(脫毒方法、病毒指數)
第9週 期中考
第10週 蘭花的繁殖 I(蘭花的無菌播種、蘭菌感染)
第11週 花的繁殖 I(營養微體繁殖,植物變異)
第12週 培植材料之準備-階段0(培植體之前處理、浸泡、生長調節劑之前處理)。
第13週 階段I及II莖頂培養及莖的培養(培養基及生長調節劑)
第14週 階段I及II 培植體體直接莖的形成(莖的形成、根的形成)
第15週 階段I及II 培植體癒傷組織器官之形成(生長調節劑之處理)
第16週 胚的形成(生長調節劑之處理)
第17週 瓶內發根後的馴化、健化植株的環境
第18週 期末考

Week 1 Course Introduction
Week 2 Introduction to laboratory equipment (production plan, cultured plant materials)
Week 3: Preparation of basic medicines (macroelements, trace elements, pH adjustment, organic acid supplementation, sugar)
Week 4: Operation of sterile operating table. (Decontamination, surface disinfection, surface contamination reduction)
Week 5 Preparation and use of culture medium (culture medium, salts)
Week 6 Factors affecting growth and morphogenesis-tissue-based factors (age of the culture)
Week 7 Pollution and Disease Control (Fungal and Bacterial Diseases, Types of Pollution)
Week 8 No virus (detoxification method, virus index)
Week 9 midterm exam
Week 10 Orchid Reproduction I (Aseptic Sowing of Orchids, Orchid Bacteria Infection)
Week 11 Flower Reproduction I (Vegetative Micropropagation, Plant Mutation)
Week 12: Preparation of culture materials - Stage 0 (pre-treatment of culture materials, soaking, pre-treatment of growth regulators).
Week 13: Stage I and II stem top culture and stem culture (medium and growth regulator)
Week 14 Stages I and II: Direct stem formation of the plant body (stem formation, root formation)
Week 15: Phase I and II: Formation of callus tissue and organs of cultured cells (treatment with growth regulators)
Week 16: Embryo formation (treatment with growth regulators)
Week 17: Acclimating and strengthening the plant environment after rooting in the bottle
Week 18 Final Exam

參考書目 Reference Books

植物組織培養與技術

校閱:楊美桂

譯者:上野洋一郎.柯俊良.陳怡菁.郭順宇.郭榮烈.陳泓志.胡威文.張傳偉



內容簡介

  本書為包括初學者在內,以想利用植物組織培養方法於研究及實際應用的人員為對象,編輯為根據正確的知識,學會培養技術而為相當的實用性。所以本書提到有關組織培養的任何項目,即如材料、器具的操作方法等有關組織培養的基本事項,以及有關器官、組織、細胞培養之基本和應用的實驗背景,實驗過程、注意事項等。



(1)本書為包括初學者在內,以想利用植物組織培養方法於研究及實際應用的人員為對象,編輯為根據正確的知識,學會培養技術而為相當的實用性。



(2)所以本書提到有關組織培養的任何項目,即如材料、器具的操作方法等有關組織培養的基本事項,以及有關器官、組織、細胞培養之基本和應用的實驗背景,實驗過程、注意事項等。品牌
藝軒圖書
語言
中文
遮蓋類型
平裝
國際標準書號
9576165849
出貨地
新北市新店區

Plant tissue culture and technology

Proofreader: Yang Meigui

Translator: Yoichiro Ueno. Ke Junliang. Chen Yijing. Guo Shunyu. Guo Rongli. Chen Hongzhi. Hu Weiwen. Zhang Chuanwei



Content introduction

This book is aimed at people, including beginners, who want to use plant tissue culture methods for research and practical applications. It is edited to be very practical for learning cultivation techniques based on correct knowledge. Therefore, this book mentions any item related to tissue culture, that is, basic matters related to tissue culture such as materials and operating methods of equipment, as well as experimental background, experimental procedures, and precautions related to the basics and applications of organ, tissue, and cell culture.



(1) This book is intended for people, including beginners, who want to use plant tissue culture methods for research and practical applications. It is edited so that people can learn culture techniques based on correct knowledge and is of considerable practicality.



(2) Therefore, this book mentions any item related to tissue culture, that is, basic matters related to tissue culture such as materials and operating methods of equipment, as well as experimental background, experimental procedures, and precautions related to the basics and applications of organ, tissue, and cell culture. Brand
Yixuan Books
Language
Chinese
Covering type
Paperback
ISBN
9576165849
Shipping place
Xindian District, New Taipei City

評分方式 Grading

評分項目
Grading Method
配分比例
Percentage
說明
Description
期中考
midterm exam
30
期末考
final exam
30
報告
report
15
出席
Attend
25

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課程資訊 Course Information

基本資料 Basic Information

  • 課程代碼 Course Code: 8305
  • 學分 Credit: 0-3
  • 上課時間 Course Time:
    Tuesday/9,10,11[寒305]
  • 授課教師 Teacher:
    羅惠萍
  • 修課班級 Class:
    精農系2-4
  • 選課備註 Memo:
    上課地點:彰化埤頭校區
選課狀態 Enrollment Status

目前選課人數 Current Enrollment: 10 人

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