本課程是10901基因操作概論(深碗型課程) 的延續性課程，同樣將以小腦脊髓運動失調症的三型疾病基因(ataxin-3)的表達系統，作為貫穿課程操作原理及實驗的模板，將遺傳工程課程內容的三大主題設計成實作單元(如圖)，進階的遺傳工程的三大單元，以團體導向學習結合過去的研究經驗實例討論，配合動手實作及分析，期使大學部高年級學生了解如何以遺傳工程技術學來探討一個特定生物科學的問題。內容包含基因選殖基本及進階應用方法，真核細胞中的基因表達，各類真核細胞表達載體，動物細胞轉染，西方點墨法，定點突變法，基因靶向 (gene targeting)，轉殖小鼠基因型確認，遺傳工程在醫學及疾病治療的應用等。本課程將以實驗原理配合實驗實作，以過去研究成果演示及討論，來顯示遺傳工程技術學的重要實作觀念。期望能灌輸學生基本觀念，並加強實作設計經驗。This course is 10901 Introduction to Genetic Operations (deep bowl type course) The continuation course will also use the expression system of the cerebellospinal dyskinesia type 3 disease gene (ataxin-3) as a template for the operating principles and experiments throughout the course, and design the three major themes of the genetic engineering course content into practical implementations The unit (pictured), the three major units of advanced genetic engineering, uses group-oriented learning combined with past research experience case discussions, and hands-on practice and analysis to enable senior students in the undergraduate department to understand how to use genetic engineering technology to learn Explore a specific biological science question. The content includes basic and advanced application methods of gene selection, gene expression in eukaryotic cells, various eukaryotic cell expression vectors, animal cell transfection, Western dot method, site-directed mutagenesis, gene targeting, Confirmation of the genotype of transgenic mice, application of genetic engineering in medicine and disease treatment, etc. This course will combine experimental principles with experimental implementation, and demonstrate and discuss past research results to demonstrate the important implementation concepts of genetic engineering technology. It is expected to instill in students basic concepts and enhance practical design experience.
本課程由謝明麗老師以課前講義配合power point講授方式進行, 學生將閱讀及整理討論特定基因的操作。本課程將以實驗原理配合實驗設計，以實驗結果討論來表示遺傳工程技術學的重要實作觀念。期望能灌輸學生基本觀念，並加強實作設計經驗。 內容包含基因選殖基本及進階應用方法，各類載體及酵素，DNA/RNA各類分析方法，定點突變法，基因targeting等。
This course is conducted by teacher Xie Mingli using pre-class lectures and power point teaching. Students will read, organize and discuss the operation of specific genes. This course will use experimental principles, experimental design, and discussion of experimental results to present important practical concepts in genetic engineering technology. It is expected to instill in students basic concepts and enhance practical design experience. The content includes basic and advanced application methods of gene selection, various vectors and enzymes, various DNA/RNA analysis methods, site-directed mutagenesis, gene targeting, etc.
• Gene Cloning and DNA analysis (7th ed.) T.A. Brown
• 應用分子生物學 Applied Molecular Biology 修訂版 李昭鋐著
• Principles of Gene Manipulation (sixth ed.) Primrose
• Recombinant DNA (third ed.) Waston, Caudy, Myers, and Witkowski
• Gene Cloning and DNA analysis (7th ed.) T.A. Brown
• Applied Molecular Biology revised edition by Li Zhaohong
• Principles of Gene Manipulation (sixth ed.) Primrose
• Recombinant DNA (third ed.) Watson, Caudy, Myers, and Witkowski
| 評分項目 Grading Method | 配分比例 Grading percentage | 說明 Description |
|---|---|---|
| 實作練習實作練習 Practical exercises |
30 | |
| 分組報告分組報告 Group report |
30 | |
| 期末報告期末報告 Final report |
40 |
