本課程將以遺傳工程實驗原理，配合以實驗方法與結果分析，來表示遺傳工程的基本觀念，以分組學習及討論方式，並期使大三大四學生了解如何以遺傳工程技術學來探討生物科學的本質。內容包含基因選殖基本及進階應用方法，真核細胞中的基因表達，各類真核細胞表達載體，動物細胞轉染，西方點墨法，定點突變法，基因靶向 (gene targeting)，轉殖小鼠基因型確認，遺傳工程在醫學及疾病治療的應用等。

This course will use the experimental principles of genetic engineering, combined with experimental methods and result analysis, to express the basic concepts of genetic engineering. It will be studied and discussed in groups, and it is expected to enable junior and senior students to understand how to use genetic engineering technology to explore the essence of biological science. The content includes basic and advanced application methods of gene selection, gene expression in eukaryotic cells, various eukaryotic cell expression vectors, animal cell transfection, Western dot ink method, site-directed mutagenesis, gene targeting, genotype confirmation of transgenic mice, and the application of genetic engineering in medicine and disease treatment.

本課程由謝明麗老師以課前講義配合power point講授方式進行, 學生將閱讀及整理討論特定基因的操作。本課程將以實驗原理配合實驗設計，以實驗結果討論來表示遺傳工程技術學的重要實作觀念。期望能灌輸學生基本觀念，並加強實作設計經驗。 內容包含基因選殖基本及進階應用方法，各類載體及酵素，DNA/RNA各類分析方法，定點突變法，基因targeting等。

This course is conducted by teacher Xie Mingli using pre-class lectures and power point teaching. Students will read, organize and discuss the operation of specific genes. This course will use experimental principles, experimental design, and discussion of experimental results to present important practical concepts in genetic engineering technology. It is expected to instill in students basic concepts and enhance practical design experience. The content includes basic and advanced application methods of gene selection, various vectors and enzymes, various DNA/RNA analysis methods, site-directed mutagenesis, gene targeting, etc.

• Gene Cloning and DNA analysis (7th ed.) T.A. Brown
• 應用分子生物學 Applied Molecular Biology 修訂版 李昭鋐著
• Principles of Gene Manipulation (sixth ed.) Primrose
• Recombinant DNA (third ed.) Waston, Caudy, Myers, and Witkowski

• Gene Cloning and DNA analysis (7th ed.) T.A. Brown
• Applied Molecular Biology revised edition by Li Zhaohong
• Principles of Gene Manipulation (sixth ed.) Primrose
• Recombinant DNA (third ed.) Watson, Caudy, Myers, and Witkowski